Transgenic potato plants resistant to the phytopathogenic bacterium Erwinia carotovora
نویسندگان
چکیده
منابع مشابه
Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants.
Snakin-1 (SN1), a cysteine-rich peptide with broad-spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91-98% identity values. Potato plants (S. tuberosum subsp. tuber...
متن کاملResponse of endophytic bacterial communities in potato plants to infection with Erwinia carotovora subsp. atroseptica.
The term endophyte refers to interior colonization of plants by microorganisms that do not have pathogenic effects on their hosts, and various endophytes have been found to play important roles in plant vitality. In this study, cultivation-independent terminal restriction fragment length polymorphism analysis of 16S ribosomal DNA directly amplified from plant tissue DNA was used in combination ...
متن کاملThe phytopathogenic bacteria Erwinia carotovora infects Drosophila and activates an immune response.
Although Drosophila possesses potent immune responses, little is known about the microbial pathogens that infect Drosophila. We have identified members of the bacterial genus Erwinia that induce the systemic expression of genes encoding antimicrobial peptides in Drosophila larvae after ingestion. These Erwinia strains are phytopathogens and use flies as vectors; our data suggest that these stra...
متن کاملPolygalacturonase of Erwinia carotovora.
The polygalacturonase, clearly differentiated from polygalacturonic acid trans-eliminase, produced in the culture fluid of Erwinia carotovora was purified 56-fold by means of acetone precipitation, acetate butfer extraction, and two successive treatments by chromatography on carboxymethyl cellulose. The purified enzyme is specific for nonmethoxylated polygalacturonic acid. It shows an optimum p...
متن کاملExpression of a Chimeric Magainin Gene in Potato Confers Improved Resistance to the Phytopathogen Erwinia carotovora
Magainin peptides originally identified from Xenopus laevis have cytotoxic effects against a range of prokaryotic organisms without harmful effects on higher eukaryotes. The mechanism of cytotoxicity of the peptides is by disruption of membranes, which causes osmolysis. Magainin peptides are known to inhibit the in vitro growth of many phytopathogens including Erwinia carotovora, the causative ...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 1993
ISSN: 0960-7412
DOI: 10.1046/j.1365-313x.1993.03040587.x